KMID : 0364820190550020177
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Korean Journal of Microbiology 2019 Volume.55 No. 2 p.177 ~ p.179
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Draft genome sequence of humic substances-degrading Pseudomonas kribbensis CHA-19 from temperate forest soil
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Kim Doc-Kyu
Lee Hyoung-Seok
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Abstract
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Pseudomonas kribbensis CHA-19 was isolated from a temperateforest soil (mid latitude) in New Jersey, USA, for its ability todegrade humic acids, a main component of humic substances(HS), and subsequently confirmed to be able to decolorize lignin(a surrogate for HS) and catabolize lignin-derived ferulic andvanillic acids. The draft genome sequence of CHA-19 wasanalyzed to discover the putative genes for depolymerization ofpolymeric HS (e.g., dye-decolorizing peroxidases and laccaselikemulticopper oxidases) and catabolic degradation of HSderivedsmall aromatics (e.g., vanillate O-demethylase andbiphenyl 2,3-dioxygenase). The genes for degradative activitywere used to propose a HS degradation pathway of soil bacteria.
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KEYWORD
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catabolic pathway, degradative enzyme, humic acids, soil bacteria
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